Study on fluorescence and DNA-binding of palladium(II) complex containing glycine and valine

Binding of small molecules with DNA has been studied extensively since DNA is the material ofinheritance and controls the structure and function of cells [1-3]. The new Palladium(II) complex,[Pd(Gly)(Val)] ,(where Gly is glycine, Val is valine)has been synthesized and characterized by elementalanalyses, molar conductivity measurements and spectroscopic methods (FT-IR, 1H NMR, UV–Vis). Bothligands coordinate to Pd(II) center as bidentate chelates. This complex is non-ionic in nature and possesssquare planer geometry around Pd(II) metal ion. The binding properties of this complex with CalfThymus DNA (CT-DNA) have been investigated by UV–Vis absorption and competitive DNA-bindingstudies with ethidium bromide (EB) by fluorescence. The results obtained from these analyses indicatedthat this complex effectively interact with CT-DNA at low concentration. Binding constant (Kb), apparentbiomolecular quenching constant (Kq) and number of binding sites (n) for CT-DNA were calculated usingStern-Volmer equation. Also, The thermodynamic parameters (ΔHº, ΔSº and ΔGº) indicated that thevander Waals and hydrogen binding might play a major role in the interaction of this complex with CTDNA.The above mentioned physical measurements indicate that the Pd(II) complex bind to calf thymusDNA, presumably via groove binding mode. In addition, the binding forces are spontaneous owing to -ΔG◦.

کلیدواژه ها:

DNA-binding ، Pd(II) complex ، Amino acids