Geraphene oxide (GO) modulates the activity of Thermomyceslanuginosus lipase (TLL)

Enzymes are biocatalysts with high-performance under mild conditions.Lipases (triacylglycerolhydrolase, E.C. 3.1.1.3) are the enzymesthat catalyze hydrolysis of triacylglycerides into free fatty acidsand glycerol at the lipid–water interface. lipases are frequently used in various industrial process likedetergent formulations, oleochemical industry[1], biofuels, food and dairy, agro-chemical, papermanufacturing, nutrition, cosmetics and pharmaceuticals. Lipases are well known industrial biocatalystdue to their ability to carry out multitude of bioconversion reactions[2].Recently, immobilization ofenzymes has studied to improve enzymes economic efficiency such as storage capacitance,enhanceactivity and stability under different conditions,and so on. Geraphene oxide has shown promisingapplication as support for heterogeneous catalysts[3][4]Graphene oxide (GO), possess large specificsurface area, the abundant oxygen-containing surface functionalities, such as, epoxide, hydroxyl andcarboxylic groups, and high water solubility. These features provides an ideal substrate for study enzymeimmobilization. However, few studies about the interaction of biomacromolecules, such an enzymes, toGO have been reported to date.We demonstrated that the lipase from TLL adsorption immobilization onthe GO sheets could take place readily without using any cross-linking reagents and additional surfacemodification. The catalytic properties of Go-Lipase nanocomposite shows its possible application andstorage capability.Keyword:Enzyme. Lipase