Phylogenetic comparison of Alburnoides samii in Barkili and Chalkeh rivers (Caspian Sea basin) compared to other Alburnoides

The genus Alburnoides is one of the most important Cyprinid genera. This genus is vastly distributed in south-west Asia and Europe. Alburnoides samii is distributed in south of the Caspian Sea basin. The analyzed fish were collected from Barkilirud and Chalkehrud Rivers using back-pack electro-shocker in 2016. In this study the sequences of Cytochrome Oxidase Subunit one (COI) were produced. The produced haplotypes were compared to similar sequences in NCBI database. The Maximum Likelihood (ML), Maximum Parsimony (MP), and Neighbour-Joining (NJ) phylograms produced with 500 bootstrap replicates were similar in topology. On the phylogram A. samii, A. tabarestanensis, A. idigensis, A. namaki, and A. coadi were nested in a monophyletic group with 92 percent boot-strap support. Alburnoides samii has the highest K2P genetic distance to A. eichwaldii (4%) and has the least genetic distance to A. idigensis and A. nicolausi (2.6%). The highest K2P genetic distance was between A. tabarestanensis and A. eichwaldii (5.2%) and the least K2P genetic distance was between A. namaki and A. coadi (0.5%). Genetic distances among Alburnoides spp. in GENBANK were 0.59 to 5.46%.

کلیدواژه ها:

A. samii ، Caspian Sea. Barkili and Chalkeh rivers ، COI gene ، genetic distance