Vitellogenin assay by Enzyme Linked Immunosorbant Assay (ELISA) as a biomarker of endocrine disruptor chemicals (EDCs) pollution

There is increasing evidence that many xenobiotic chemicals (called as endocrine disruptor chemicals EDCs) through interfering with endocrine system, have the capability to induce developmental and reproductive abnormalities in humans and animals. The yolk protein precursor vitellogenin (VTG) has proved to be a simple and sensitive biomarker for assessing exposure of fish to EDCs. In this study we have developed quantitative enzyme linked immunosorbent assays (ELISA) for VTS in common carp. The working range of the ELISA was 11.25 to 2,000 ng/ml (75-25% specific binding/maximum antibody binding [B/B0]) with a 50% B/B0 intra- and interassay variation of 3.9% (n = 10) and 12.5% (n = 30), respectively. This ELISA is capable of detecting VTG as low as 6 ng/ml, and can accurately detect VTG in even 10l of plasma. The ELISA was applied to measurement of VTG production by male carp exposure to ethynylestradiol. The amount of VTG produced in plasma of exposed fish increased in logaritmic order comparing to the control group.

کلیدواژه ها:

Immunoassay ، ELISA ، Endocrine Disruptor Chemicals ، VTG