Application of salicylic acid and sulfuric acid in canola affects Hsp gene expression in Brevicoryne brassicae (Hemiptera: Aphididae)

The current research was carried out in green house condition in order to investigate the effect of application of sulfuric and salicylic acids in Okapy canola cultivar on Hsp gene expression in the cabbage aphid. The heat shock ( Hsp) proteins present in all organisms and protect them against the various stresses in environment. The experiment was a factorial design with salicylic (SA) and sulfuric acids as factors each with two levels (0 and 75 µM for SA; 0 and %0.05 for the latter), with 5 replications. Canola seeds were soaked in 75 µM SA, 24 hours prior to planting in pots with 25 cm diameter and 5 Kg field soil. Application of %0.05 sulfuric acid was done in 4-5 leaf stages during irrigation. Approximately, 30 aphids from each replication were sampled after 30 days feeding on the plants. Then total RNA was extracted and cDNA was synthesized. Quantitative PCR was carried out on Hsp70 gene and 16S rRNA as a standard gene. The results showed a significant and very significant difference in gene copy number in aphids fed on SA and sulfuric acid treated plants, respectively. This indicates that salicylic acid and dilute sulfuric acid might be used as agents for increasing plant resistance against the cabbage aphid.

کلیدواژه ها:

acquired resistance ، cabbage aphid ، Hsp70 ، salicylic acid ، sulfuric acid