Production of recombinant construction pBudCE4.1IFNβ-1a including recombinant Interferon beta gene and transfection into HEK293cell line

سال انتشار: 1393
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 770

متن کامل این مقاله منتشر نشده است و فقط به صورت چکیده یا چکیده مبسوط در پایگاه موجود می باشد.
توضیح: معمولا کلیه مقالاتی که کمتر از ۵ صفحه باشند در پایگاه سیویلیکا اصل مقاله (فول تکست) محسوب نمی شوند و فقط کاربران عضو بدون کسر اعتبار می توانند فایل آنها را دریافت نمایند.

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

CIGS13_0239

تاریخ نمایه سازی: 7 بهمن 1393

چکیده مقاله:

Interferons(IFNs) are a group of secretory cytokines with a low molecular weight which possess broad range of biological effects, including antitumoral properties, adjust the immune system and regulate cell differentiation. Human IFNβ is afibroblastic glycoprotein produced in response to viral infections. In medicine, IFNβ used in order to autoimmune diseasestreatment such as multiple sclerosis, cell proliferation and angiogenesis inhibition. IFNβ has two analogs. Non-glycosylatedform, IFNβ-1b, and glycosylated form, IFNβ-1a. Object: According to the transient expression of IFNβ and its medical necessity, changes the gene in order to produce more and better quality protein, will be discussed. Method:In this study, the expression level of recombinant human IFNβ-1a gene including kozak conserved sequence and targeted mutations in 27 and 101 codons in gene coding region at both mRNA and protein levels will be examined. PBudCE4.1 is selected vector for this study which designed for the same expression of two genes in mammalian cell lines. HEK293 usedfor transfection of our designed vector. This selected cell line is known as one of the best choices for recombinant gene expression, producing variety of viral vectors and vaccines aims. In this examination, Real time PCR and protein diagnostic techniques such as Dot blot and Elisa will be considered. Conclusion: Low biological activity of IFNβ-1b produced in Ecoli and different physicochemical and immunogenicity properties compare to natural form, reveal the importance of protein glycosylation. In addition, mutations applied in order to increase stability, binding affinity and protein proper folding will be important

کلیدواژه ها:

نویسندگان

Rahele Norouzi

Division of Genetics, Department of Biology, Faculty of Science, University of Isfahan, Isfahan, IR Iran

Zohreh Hojati Najaf abadi

Division of Genetics, Department of Biology, Faculty of Science, University of Isfahan, Isfahan, IR Iran