Study of correlation between SFRP1 & SFRP2 hypermethylation with genetic mutations of FLT3,NPM1 and immunophenotypes of leukemic cells in patients with acute myeloblastic leukemia

سال انتشار: 1393
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 991

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شناسه ملی سند علمی:

CIGS13_0521

تاریخ نمایه سازی: 7 بهمن 1393

چکیده مقاله:

Background: AML is a clonal disorder of hematopoietic stem cells that is associated with the infiltration of blasts in the peripheral blood and bone marrow. In recent years, The role of epigenetic abnormalities such as promoter methylation of tumor suppressor genes Including family genes SFRPs (Secreted frizzled related protein) has been shown in thepathogenesis of cancers. This study evaluates the correlation between SFRP1 and SFRP2 hypermethylation with genemutations of FLT3, NPM1 and immunophenotypes of leukemic cells. Materials and Methods: In peripheral blood from 43 patients afflicted with de novo AML, isolated DNA was treated with sodium bisulphite and analyzed by methylation-specific polymerase chain reaction (MSP). NPM1 and FLT3-ITD mutationsdetection by capillary electrophoresis. Fisher exact test and SPSS21was used for statistical data analysis.Results: SFRP1 hypermethylation had Significant association with CD34 (P 0/=045) and CD14 (P =0/046) expression.FLT3-ITD and NPM1 mutations among 43 tested patients found in 8 (18.6℅) and 6 patients (13.9℅), respectively. There was no difference in the incidence gene mutations of FLT3 & NPM1 between patients with and without SFRP hyper methylation. Conclusion: Based on these results, hypermethylation SFRP1 is associated with increased expression of CD34 and CD14 antigens. However, further studies on a large group of patients are necessary to confirm our findings.

نویسندگان

Ali Ghasemi

MSc Student, Department of Hematology, School of Allied Medical Sciences, Tehran university of Medical Sciences,Tehran, Iran

Shahrbanoo Rostami

Hematology-Oncology and Stem cell Transplantation Research Center, Tehran university of Medical Sciences, Tehran, Iran

Fatemeh Nadali

Associate Professor, Departement of Hematology, School of Allied Medical Sciences, Tehran university of Medical Sciences, Tehran, Iran

Abbas Ghotaslou

MSc Student, Department of Hematology, School of Allied Medical Sciences, Tehran university of Medical Sciences,Tehran, Iran