CCND1 expression profile in different breast cancer cell lines

Introduction: Cyclin D1 extensively established as an oncogene with an imperative pathogenetic role in breast cancer. Up-regulation of this gene is driven by chromosome translocation, gene amplification, polymorphism, post-transcriptional regulation and protein stability. This investigation aims at exploring the transcriptional profiling of this oncogene in various breast cancer cell lines.Methods: The transcript levels of CCND1 in 41 breast cancer cell lines obtained via GENT database which measured by Affymetrix U133A platform. CCND1 expression evaluated based on transcriptional profiling including tumor source (primary, metastasis), subtype, pathology and ER, PR and HER2 receptors.Results: Elevated level of D1 transcript observed in luminal, basal A and basal B subtypes, respectively. Compared to primary tumor, metastasis breast cancer cell lines revealed higher expression of D 1cyclin. Ductal carcinoma, carcinoma and adenocarcinoma cell lines demonstrated the higher mRNA levels in the same order. Moreover, greater expression identified in ER-, PR- and HER2+ cell lines.Conclusion: Up-regulation of CCND1 oncogene associated to the late stages of breast cancer and estrogen receptor status. The compilation of breast cancer molecular profiles defines cell lines suitable to uncover potential oncogenes, or provides a resource for better cancer prognosis.

کلیدواژه ها:

CCND1 ، Transcriptional profiling ، Breast cancer cell lines ، GENT