Poly (lactide-co-glycolide)/ Bioactive Glass Nanoparticles/Hydroxyapatite Nanoparticles Composite Coating with Osteogenic and Antibacterial Effects on Titanium

The solvent casting process was used to coat titanium substrates with poly (dl-lactic-glycolic acid), 58S bioactive glass nanoparticles (BGN), and hydroxyapatite nanoparticles (HAN) with different weight ratios (90:5:5, 85:7.5:7.5, and 80:10:10). 58S BGN with antibacterial activity which was synthesized via so-gel technique was used in the coating preparation. BGN and HAN were used in equal ratio in each composition. Techniques such as X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), Zetasizer, and X-ray fluorescent (XRF) were utilized to characterize the prepared BGN. Cytotoxicity of the BGN was evaluated using mouse fibroblast L929 cell line. Coating surface characteristics were analyzed with EDS, SEM. The chemical compositions of the prepared BGN was as predicted and the particle size of the BGN with an amorphous structure mainly ranged over 40-90 nanometers. It showed no cytotoxicity although it induced some cytotoxicity at 24 and 48 hours incubation. By 72h, 58S showed significantly greater viability and proliferation than the control did. Results showed that polymer can not surround the nanoparticles in the concentrations more than 10 wt%. Using 10 wt% nanoparticles in the coating provided a uniform coating with some nanoparticles aggregates. Cross-section images of the prepared coating showed good bonding to the substrate. Further investigations are necessary to evaluate the role of such coating in orthopedic applications.

کلیدواژه ها:

Composite coating ، Osteogenic ، ، Antibacterial activity ، Nanoparticles ، Cytotoxicity