Application of RGO-Chitosan Nanocomposite for Fabrication Sensitive Aflatoxin B1 Electrochemical Aptasensor

Mycotoxins are the secondary metabolite product of fungi and molds which are created on the agricultural products prior to or after harvest and during transportation and preservation. Mycotoxins are categorized as the most cancerogenic materials according to the IARC categorization [1]. One type of mycotoxins are the aflatoxins. Main types of aflatoxins areG1, B2, B1 and G2 amongst which aflatoxins B1 are known as the most toxic aflatoxin.Detection of aflatoxins B1 has attracted the attention of many researchers because it is highly toxic and prevalent[2]. Therefore, in this work, a sensitive electrochemical aptasensor was developed for detection of aflatoxin B1. Various RGO-chitosan nanocomposites were synthesis and used for immobilization of aflatoxin B1 aptamer molecules on the surface ofglassy carbon electrode (GCE). Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), Fourier transform infrared spectroscopy (FT-IR), scaning electron microscopy (SEM) and transmission electron microscopy were used for characterization offabricated aptasensor. In the present work the effect of various variables such as glutaraldehyde linker concentration, glutaraldehyde incubation time, capture probe concentration, capture probe incubation time, aptamer probe concentration, aptamer probeincubation time and nanocomposite composition were investigated. In optimum conditions very sensitive aflatoxinB1 electrochemical aptasensor was fabricated. In the peresent work elctrochemical impedance spectroscopy was used for detection of aflatoxin B1. Thefabricated impedimetric aflatoxinB1 aptasensor showed very good analytical parameter such as high sensivity, low detection limit, wide linear range and high selectivity. The resulted aptasensor can offer arsenic detection in environmental and other applications.

کلیدواژه ها:

Redused Gerephene oxide ، Aptasensor ، AflatoxinB1 ، Electrochemical Impedance Spectroscopy ، Electrode