Voltammetric determination of N-acetyl-L-cysteine at the surface of glassy carbon electrode modified with Prussian blue nanoparticles

N-Acetyl-L-cysteine (NAC) is an acetylated derivative of the L-cysteine, as an amino acid. NAC is commonly used as pharmaceutics. It has first been managed as a mucolytic agent reducing the viscosity of pulmonary secretions in chronic respiratory illness as well as an antidote for hepatotoxicitydue to acetaminophen overdose. It has also been efficient in the treatment of Sjogren’s syndrome, smoking cessation, influenza, hepatitis C, and myoclonus epilepsy [1]. Another less expanded chemical property of NAC concerns its antioxidant activity. As a matter of fact, NACconstitutes an excellent source of sulphydryl groups (SH), which is converted in organism into metabolites able to stimulate the synthesis of reduced glutathione (GSH). It also acts as ascavenger of free radicals and reactive oxy- gen species (ROS), consuming directly super oxide anion [2], or hypochlorous acid [3]. This work describes the electrocatalytic properties of a glassy carbon electrode (GCE) modified with poly(o-phenylenediamine) (PoPD)/Prussian blue (PB)nanoparticles (NPs) for the electrocatalytic oxidation of NAC. The stepwise fabrication processof PBNPs-modified PoPD/GCE was characterized by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy. The mechanism and kinetics of the catalytic oxidationreaction of NAC were monitored by cyclic voltammetry and chronoamperometry. Some important advantages such as simple preparation, fast response, good stability and reproducibility of the sensor for the voltammetric determination of NAC were achieved.

کلیدواژه ها:

N-Acetyl-L-cysteine ، Prussian blue nanoparticles ، Poly (o-phenylenediamine) ، Glassy carbon electrode