Identification and Validation of UGE1 Candidate Gene Associated to Drought Stress in Plants by Gene Expression Analysis

Drought stress is one of mainchallenges in crop production [1]. The research was performed to identify and validate Uge1 genes involved in drought stress using gene expression data analysis which wasdownloaded from NCBI database. We considered three datasetsrelated to three plants, barley, tomato, and soybean, to start the analysis of gene expression data [2,3]. Data analysis indicated that there are 258 genes in which at least two datasets had a significant change to drought stress (Fold Change> 2 and p-value<0.05). 18 common genes were identified which were regulatory factors based on plantfd database. We chose Uge1 geneto validate. We studied the expression of this gene in three plants (barley, soybean, and tomato) under five drought stress levels (10, 15, 20, 25, and 30 percent). It was conducted based on completely randomized factorial design. The first factor of drought stress had 5 irrigation levels of 10, 15, 20, 25 and 30% of field capacity, and the second factor included 3 different plants of barley, tomato and soybean [4,5]. After treatment of drought stress on seedling stage, the leaf tissue of plants wasexcited for RNA extraction. Gene expression was determined by RealTimePCR [6]. According to the results, for Uge1 gene expression, the highest and the lowest expression valuewere observed in tomato and soybean, respectively. In drought stress factor, maximum and minimum gene expression value were observed in the levels of 25% and 10%, respectively. This experiment was carried out at institute of Biology and Technology in University of Zabol.

کلیدواژه ها:

RT-PCR ، Uge1 ، Drought stress ، Gene expression