Effect of aqueous-methanolic extract of Artemisia absinthium on Erythromycin toxic dosage on gold fish

Objective: Erythromycin is an antibiotic which is effective on gram-positive organisms and its antibacterial action maybe inhibitory or bactericidal for susceptible organisms, but use of toxic dose of erythromycin may result in severe hepatic necrosis. In this research protective effect of aqueous-methanolic extract of Artemisia absinthium on Erythromycin toxic dosage (400mg/kg/bw) induced hepatotoxicity was evaluated on goldfish. Method & Materials: 21 gold fish performed for this experimental study which divided into 3 groups (n=7), at the first group we administered drug solvers as a placebo and in the second group we administered a toxic dose of erythromycin (400mg/kg/bw), and in the third group we administered aqueous-methanolic extract of Artemisia absinthium with dose of (500mg/kg/bw) 4 hours prior of administration of erythromycin(400mg/kg) for 21 days, (all drugs administered orally with gavages). Results & Conclusion: At the end of week 3 after bleeding from heart the serum was separated and the rate of ALT, AST, ALP, LDH, and Albumin activities were determined in the plasma of fish and results recorded in special forms. After 21 days it was found that Erythromycin over dose (400mg/kh/bw) caused liver damage as evident by statistically significant (P>o.05) increased in plasma activities of ALT, AST, ALP, LDH, and Albumin. Oral administration of aqueous-methanolic extract of Artemisia absinthium at a dose of (500mg/kg/bw) significantly reduced the toxic effects of erythromycin. From the observations, the conclusion drawn is that aqueous-methanolic extract of Artemisia absinthium stabilized the hepatic frame against the toxicity of erythromycin.

کلیدواژه ها:

Antibiotic ، Erythromycin ، Hepatotoxicity ، Artemisia absinthium ، Gold fish