Relationship of Malondialdehyde and Lipid profile with Plasminogen Activator Inhibitor -1 (PAI-1) gene polymorphism in Acute Coronary Syndrome

Introduction: Acute Coronary Arteries are caused by atherosclerosis. Studies have shown that there are several factors that are closely related to the development and progression of the disease, which include cell-binding molecules such as Plasminogen Activator Inhibitor-1 (PAI-1) and lipid peroxidation. In this study, we aimed to investigate the relationship between PAI-1 (4G/5G) gene polymorphism with oxidative stress markers such as malondialdehyde (MDA) and lipid profile.Methods: In this study, 90 subjects contain 45 patient groups and 45 control groups were considered. Blood samples were taken from all contributors. DNA genome was extracted from the blood and for evaluation of PAI-1 (4G/5G) polymorphism was used from special primer. Polymorphism at - 675 position in the PAI-1 promoter by the PCR-RFLP method were detected. The concentration of MDA in serum samples was evaluated by spectrophotometric method and based on the reaction of thiobarbituric acid. Lipid profile was also measured based on routine laboratory methods.Results: Serum level of MDA was significantly different in both the patient and control groups. Heart Risk factors such as cholesterol and triglyceride were significantly different between the patient and control groups. The serum HDL level in the patient group was lower than the control group. The distribution of the PAI-1 genotype was that the frequency of the 4G/5G genotype in the patient group Compared with the control group, it was at a higher level (77.8% in the patient group and 22.2% in the control group). In Serum HDL level there was a significant difference between 4G/4G with 4G/5G genotype (p <0.001). In Serum levels of cholesterol, triglycerides and LDL there were significant differences between the three genotypes (4G/4G, 4G/5G and 5G/5G).Conclusion: 4G/5G polymorphism of PAI-1 gene seems to be a good indicator to evaluate fibrinolytic activity and this polymorphism Causes and inhibits fibrinolytic activity Due to increased plasma levels of PAI-1, Therefore, the risk of cardiovascular disease increases. The results of this study indicate that this genetic change may cause increase cholesterol, LDL or decrease HDL.

کلیدواژه ها:

PAI-1 gene polymorphism ، MDA ، Acute Coronary Syndrome