Effect of hydro alcoholic extract of Nigella sativa on Caspase3 gene expression in MCF7 cell line

There are problems such as treatment failure, drug resistance, heavy costs and other problems related to cancer treatment, which cause the plants to attract the interest of many researchers, because they have no side effects. In this study effect of Nigella Sativa on, caspase-3 gene expression,one of genes inducing of apapotosis was studied. First Nigella sativa was extracted by 70% ethanol and concentrations of 200 and 400 μg/ml were prepared and MCF-7 cell lines treated by extractions. Then cells harvested on 24, 48 and 72 hours after treatment. Expression of caspase-3 was evaluated by Real-time PCR .The results showed that caspase-3 gene expression in MCF-7 cells was not changed significantly by Nigella sativa extract. As the concentrations of extract used in this study had no effect on caspase-3 gene, it seems that other concentrations of Nigella sativa was effective or other genes in apoptosis pathway were affected.

کلیدواژه ها:

Breast cancer ، Nigella sativa ، Drug resistance