FGF10:typeIII epithelial mesenchymal transition and invasion in breast cancer cell lines

Introduction: Epithelial mesenchymal transition (EMT) is a process related to the initiation of cell migration and metastasis. EMT categorized into three types: type 1 in gastrulation, 2 in wound healing and 3 in metastasis. Because of some similarities between procedures during gestational life and cancer, investigations about possible role of dysregulated molecules, which are responsible for organogenesis and growth of cells in fetus, on invasion and cancer metastasis is very important. FGF10 is a protein in type I EMT. There are some evidences showing correlations between the expressing levels of FGF10 and invasion of cancer cells. this study show the effect of these cell-line behaviors is directly related to FGF10 through regulation of EMT mechanisms in various types of signaling pathways.Methods: Gene regulation by siRNA and recombinant protein. Western blot, MTT, Colony agar, Migration, Wound healing and Apoptosis assay. Real Time PCR.Results: Positive effect of FGF10 on proliferation, tumorgenesis and wound healing ability of cells. Invasion and migration ability of cells dependents on FGF10 production. Apoptosis is decreased under FGF10 protein. EMT induction factors change under FGF10 effects and initiate EMT program. Conclusion: Our data suggested that if FGF10 is dysregulated in cancer, it can act as a wild agent involving cell proliferation, immortality, tumorigenesis and EMT followed by invasion and metastasis.