Studying the effect of the aqueous and Acetone extract of the Nigella Sativa on inhibition, survive and growth rate and the MCF-7 Cell grade of keratin

Introduction: Breast Cancer is the second reason of death rate of the cancers in women. The probability of catching the invasive breast cancer by women in life time is 1 in 8 (13%). The problems like the failure of treatment, resistance to the drugs, the costliness and other problems related to cancer treatment has made the attentions concentrated on the drug plants which have less side effects in comparison to the chemical drugs. In this research the effect of the aqueous and Acetone extract of the Nigella Sativa on inhibition and survive rate and the MCF-7 Cell grade of keratin is studied.Methodology: 50 grams Nigella Sativa was put in two flat bottom balloons and 300 ml distilled water and 300 acetone was put in both balloons. They were put on the mixture using magnet in 0 degrees for 25 hours and then they were passed through the filter and then the water and acetone was extracted using rotary. Finally a paste type brown material was accessed. The cancerous cells of this study were provided from Iran Pastor Institute. The breast cancer cells (MCF-7) were planted in flasks holding RPMI 1640 planting environment including 10% FBS in incubator 37ᵒc wet space along with 5% CO2. Then they were transferred to the 96 partition plates of 104*2 cell concentration in each well. And were treated with different aqueous and acetone extract of Nigella Sativa (100, 200 and 400 μg/ml) for 24, 48 and 72 hours. The effect of the extract on survive rate of the cells was studied via coloring by Trypan Blue method.Findings: The results showed that increasing the density and the time of the extract decreased the survive rate of the cells in comparison to the control group meaningfully. Conclusion: According to the findings of the research about the aqueous and acetone extract, Nigella Sativa holds anti-cancer effects on the breast cancer cells (MCF-7).