Effect of Collagen on the synthesis of Calcium Phosphate Nanoparticles

Collagen and non-collagenous proteins have an important role in the formation of mineral constituent of bone matrix. In this research, the effect of collagen on the morphological and phase characteristics of hydroxyapatite nanoparticles was investigated. The synthesis reaction was initiated by mixing H3PO4 as phosphorous source and CaCl2 as calcium source in presence of Collagen Type 1. Collagen concentration in suspension and Ca to P ratio was 1% and 1.67 respectively. The samples (with collagen and without collagen), heat treated at 600 0C were characterized by X-Ray diffraction (XRD), Fourier transformation infrared (FTIR) and Scanning electron microscopy (SEM). More smaller and flake-like shape particles were observed in the SEM images of sample synthesized in the presence of collagen compared to the control sample which was constituted of larger granular particles. The XRD and FT-IR results revealed the powders were composed of hydroxyapatite and octacalcium phosphate. P-O and OH characteristic peaks were identified in FT-IR spectra. It seemed the sample synthesized in the presence of collagen was less crystalline.

کلیدواژه ها:

Collagen Type I ، Hydroxyapatite ، nanoparticles