Distribution of Aminoglycoside Resistance Genes Among Acinetobacter baumannii Strains Isolated From Burn Patients in Tehran, Iran

Introduction and Objectives: Acinetobacter baumannii among the most common opportunistic pathogens associated with nosocomial infections, particularly in burn patients. Hospital Infections caused by these bacteria are difficult to treat. So the aim of the present study investigated the prevalence of genes encoding aminoglycoside-modifying enzymes in A. baumannii strains isolated from burned patients in Tehran, Iran Materials and Methods: This study was conducted on 100 A. Baumannii isolates collected from Shahid Motahari hospital in Tehran. The age range of the population in this study was assessed 2 to 90 years. Antibiotic susceptibility testing was performed by disk diffusion according CLSI guidelines. Also use of PCR and sequencing for the presence of genes aphA6, aacC1, aadA1 and aad B were studied.Result: A total of 100 strains studied almost all isolates to ceftriaxone, ciprofloxacin and ceftizoxime were resistant (100%), whereas the highest susceptibility was observed towards colistin sensitivity (100). Percent, resistance to various aminoglycoside: gentamycin (93%), amikacin (90%), tobramycin (87%), respectively. aphA6 gene in 65 cases (81%), aad B gene in 72 (0.9%) cases, aacC1 gene in 21 patients (26%) and aadA1 gene in 37 (46%) of the isolates were identifiedConclusion: Our results showed that A. baumannii strains resistant to antibiotics used is increasing, so the implementation of appropriate programs to prevent the spread of the bacteria in the Shahid motahari hospital is necessary.

کلیدواژه ها:

Aminoglycoside resistance genes ، Acinetobacter baumannii ، Multidrug resistance