Echinochandin resistance in Candida parapsilosis sensustrico: Role of alternations in CHS3, FKS1 and Rho gene expressions

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 426

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شناسه ملی سند علمی:

ICCM13_023

تاریخ نمایه سازی: 25 آبان 1398

چکیده مقاله:

Background and Objectives: Candida parapsilosis has emerged as an important pathogen which accounted for 24.4% of all episodes of invasive candidiasis in Iran. However, the rate of resistance of C. parapsilosis to echinocandins remains unexplored in Iran. The main aim of the study was to investigate the susceptibility pattern and the possible mechanism of resistance in echinocandin-resistant clinical isolates of C. parapsilosis. Materials and Methods: One hundred and five isolates of C. parapsilosissensustricto were subjected to antifungal susceptibility testing against Caspofungin (CSP), Anidulafungin (ANF) and Micafungin (MCF) using the broth microdilution reference method. The sequences of CpERG3 andalso CpFKS1 genes were analyzed using MEGA6 software and alternations in CHS3, FKS1 and Rho gene expressions were evaluated using Real-time PCR. The REST® software was applied to analyze the results. Results: The rate of cross-echinocandin resistance was reported as 2.8%. No substitutions was detected in Fks1p except for the naturally occurring P660A amino acid substitution observed in isolates both with high and also low MIC values. The amino acid substitution G111Rwas not found in Erg3p, either. The expression of Rho and FKS1 genes was significantly increased while CHS3 gene showed no alternation after echinocandin exposure. Conclusion:Alternations in the expression of some key genes may be responsible for echinocandin resistance among C. parapsilosis isolates. Understanding the mechanisms responsible for drug resistance in C. parapsilosis is not only crucial for the development of new antifungals but is also important in choosing appropriate antifungals for patients at the earliest stages.

نویسندگان

Maryam Moazeni

Invasive Fungi Research Center, Mazandaran University of Medical Sciences, Sari, Iran;

Iman Haghani

Department of Medical Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran;

Amirhossein Davari

Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran;

Mojtaba Nabili

Facaulty of Medicine, Sari Branch, Islamic Azad University, Sari, Iran