Molecular and Antigen/antibody Detection of Bovine Rotavirus (BRV), Bovine Viral diarrhea (BVD) in Diarrheic Stool Samples in Iran

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 401

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شناسه ملی سند علمی:

ICCM13_161

تاریخ نمایه سازی: 25 آبان 1398

چکیده مقاله:

Background and Objectives: Calf scours play a major role in economic losses of animal farming industry around the world since causing approximately more than 50% of deaths in unweaned diarrhea calves. The main goals of present study were simultaneous investigation of prevalence of BRV and BVD in dairy calves by two methods in some provinces of Iran. Moreover, comparing the diagnostic efficacy of ELISA and RT-PCR as well as season and geographic area on amount of BRV and BVD positivity. Materials and Methods: A total of 73 diarrheatic fecal samples from E.coli infection negative calves up to one week and under a month dairy Holstein cattle herds of some provinces of Iran during autumn and winter seasons were collected. After preparations, samples were subjected to antigen detection by ELISA (Enzyme Linked ImmunoSorbent Assay) and RNA Extraction by semi-manual approach and CDNA synthesis for PCR amplification. Then, gel electrophoresis, sequencing for confirmation of bands and submission of sequence were followed. Finally, statistical analyses were done by SPSS Ver. 22. Software Results: From the total of 73 calves diarrheatic samples, 28 (38.3 %) and 31 (42.4%) were positive for presence of RV and 1 (1.36 %) and zero for BVD by ELISA and RT-PCR, respectively. The Kappa coefficient (for ELISA and RT-PCR) showed significant differences between two tests and they were not in agreement The distribution of the positive samples by city showed 80%, 52.6% and 50% samples from Eslamshahr, Ghazvin and Hamedan were RV positive, respectively, among diarrhoeic bovine calves. So, the prevalence of BRV in these cities/provinces is high. Also, the prevalence rates were significantly more by ELISA in autumn and by RT-PCR method in Winter. In case of BVD, only ELISA positive sample was reported at autumn. Conclusion: The results of the present study indicated that rotavirus has a high incidence in diarrhetic calves as well as in winter season. BVDV except one sample in ELISA assay, has not any other positive in ELISA and PCR. RT- PCR is considerably rapid, sensitive and more accurate technique than ELISA for identification of RV and BVD.

نویسندگان

Mohammad Mehdi Ranjbar

Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran.

Soodeh Enayati

Department of Biotechnology research and science branche, Islamic Azd University, Tehran, Iran

Hamzeh Ghobadian

Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran.

Mehdi Mehri

Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran.