Cloning, Expression, and Computational Studying of E. coli L-Asparaginase II to Alter Substrate Specificity
سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 401
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شناسه ملی سند علمی:
ICCNRT02_072
تاریخ نمایه سازی: 30 آذر 1398
چکیده مقاله:
Asparaginase is an enzyme that hydrolyzes the amide bond in L-asparagine and similar amides and possesses expandable anti-cancer properties. In this study, the E. coli L-asparaginase II gene was cloned and the recombinant protein was expressed and its enzymatic activity was measured. To find a mutant with altered substrate specificity, two active site residues were chosen as the starting point for mutagenesis and the effect of the mutation on the enzyme s structure was studied by computational methods
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نویسندگان
Hamid R Kalhor
Department of Chemistry, Sharif University of Technology, Tehran, Iran
Daniel Yousefi
Department of Chemistry, Sharif University of Technology, Tehran, Iran