Application of the Taguchi Method to optimize the process conditions in the production of 2,3-Butanediol by Klebsiella pneumoniae PTCC1290

In this study, optimal operating parameters of 2,3-Butanediol production using Klebsiella pneumoniae under submerged culture conditions are determined by using Taguchi method. The effect of different factors including medium composition, pH, temperature, mixing intensity and inoculum size on 2,3-butanediol production was analyzed using the Taguchi method in three levels. Based on these analyses the optimum concentrations of acetic acid and succinic acid were found to be 0.5 and 1.0 ( %w/v) respectively. Furthermore, optimum values for temperature, inoculum size, pH and the shaking speed were determined as 37 °C, 8 (g/L), 6.1 and 150 rpm respectively. The influence of the trace elements on 2,3-butanediol production was studied and it was shown that their addition to the medium was not necessary. Maximum 2,3-butanediol concentration under optimum conditions was obtained as 15.973 (g/L) after 48 hrs of cell cultivation.

کلیدواژه ها:

2 ، 3-Butanediol production ، optimization ، Klebsiella pneumoniae ، Taguchi method