Preventive effect of 25Mg-PMC16 Nanoparticles on Cytotoxicities of Energy Metabolism Inhibitors in Isolated Lymphocytes of Rat Blood

The magnetic isotope effect of 25Mg2+, the only magnetic magnesium isotope (+5/2 nuclear spin, 0.85 Bohr magneton magnetic moment, 10% natural abundance), has been found to be an essential, overactivating, element in the magnesium-dependent ATP production control processes. Being Mg2+-dependent processes, both substrate and oxidative phosphorylation pathways might be activated up to 2.5-fold more efficiently by milimolars of 25Mg2+ as compared to non-magnetic 24Mg2+ (0 spin, 78% natural abundance) and 26Mg2+ (0 spin, 11% natural abundance) isotopes (1).A low toxic (LD50=896 mg/kg, i.v., rats), amphiphilic (430 mg/ml water, pH 7.40), membranotropic and cluster forming 1.8-2.0 nm fullerene-C60 based nanoparticles has been recently designed for correction of hypoxia-induced ATP depletion (2). This product, which possesses a marked cationite properties, is the iron containing porphyrin monoadduct of a classical Buckminster fullerene, buckminster-fullerene(C60)-2-(butadiene-1-yl)-tetera(o-γ-aminobutyryl-o-phtalyl)ferroporphyrin. Hereafter, we refer it to Porphylleren-MC16 or, in brief, PMC16 (2).This smart nanocationite with membranotropic properties release the overactivating cations only in response to the intracellular acidosis (2).