Design of a peptide containing single chain variable fragment of anti HER2 antibody and evaluation of its Nano particles in targeted gene delivery

Peptide based gene carriers containing targeting motif are among the most promising non-viral vectors for targeted genedelivery to cancer cells. We have engineered a new fusion peptide using recombinant technology with the purpose ofovercoming the cell barriers to targeted gene delivery to cancer cells. The fusion peptide is composed of two repeats oftruncated histone H1 peptide to condense pDNA, a fusogenic peptide to disrupt endosome membranes and a nuclearlocalization signal to enhance translocation of pDNA towards nucleus and a targeting motif to targeted gene delivery tobreast cancer cells.The results demonstrated that the vector can effectively condense plasmid DNA into nanoparticles with average sizes of300 nm. Overall, the transfection efficiency of the vector demonstrated that it holds great promise as a nontoxic andeffective non-viral gene carrier for targeted gene delivery to cancer cells.

کلیدواژه ها:

Gene therapy ، nanoparticles ، fusogenic peptides ، targeting motif ، luciferase reporter gene