Cloning of the surface layer gene from Lactobacillus acidophilus ATCC 4356 and its heterologous expression and purification
سال انتشار: 1393
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 478
متن کامل این مقاله منتشر نشده است و فقط به صورت چکیده یا چکیده مبسوط در پایگاه موجود می باشد.
توضیح: معمولا کلیه مقالاتی که کمتر از ۵ صفحه باشند در پایگاه سیویلیکا اصل مقاله (فول تکست) محسوب نمی شوند و فقط کاربران عضو بدون کسر اعتبار می توانند فایل آنها را دریافت نمایند.
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
ICNN05_024
تاریخ نمایه سازی: 30 آبان 1394
چکیده مقاله:
Lactobacillus acidophilus ATCC 4356, like many other bacteria, harbours a surface layer consisting of aprotein (S-protein) of 44.2 kDa organized in regular arrays. The gene encoding this protein has been cloned in Escherichiacoli and sequenced. SlpA gene consists of 1360 nucleotides that has been amplified by PCR and the fragment has beencloned in E. Coli BL21. S-layer monomers expression has been verified by SDS-PAGE and the molecular mass was thesame as predicted size. Moreover, Purification of S-layer subunits on Ni-NTA column was conducted. Sequencecomparison of S-protein of with the other S-proteins from various species shows 78% similarity with Lactobacillushelveticus ATCC 12046. Additionally, the presence of two domains has been suggested, one comprising the N-terminaltwo-thirds (SAN) and another made up of the C-terminal one-third (SAC) of S-protein. The sequence of the N-terminaldomains is changeable, while that of the C-terminal domain is highly conserved in the S-proteins of these organisms. Thecapacity of S-protein of L. acidophilus as a probiotic makes it suitable for application as an oral delivery vehicle in thefields of nanobiotechnology.
کلیدواژه ها:
Surface layer protein ، Lactobacillus acidophilus ATCC 4356 ، Cloning ، Expression ، TransmissionElectron Microscopy (TEM)
نویسندگان
E Hosseini
Department of Biological Science, Tarbiat Modares University, Tehran, Iran
R Kasra Kermanshahi
Department of Science, Alzahra University, Tehran, Iran
M Nazari
Department of Nanobiotechnology, Avicenna Research Institute, ACECR, Tehran, Iran
S Hosseinkhani
Department of Biological Science, Tarbiat Modares University, Tehran, Iran