Preparation and in-vitro Transfection Efficiency Evaluation of Modified Cationic Liposome-polyethyleneimine-plasmid Nanocomplexes as a Novel Gene Carrier

In this study, we tried to improve the transfection efficiency of these nanocomplexes by liposomemodification. For this aim, the lipopolymer prepared by the conjugation of hexylacrylate to the PEI. Liposomescomprising of lipopolymer and DOTAP were prepared in desired size. different molecular weights of linear PEI havebeen used in order to prepare modified liposome-PEI-DNA nanocomplexes. Three C/P ratios of each nanocomplexeswere premixed. Size, zeta potential and the DNA condensation ability of these complexes were determined, and at theend, the transfection efficiency and cell cytotoxicity of prepared vectors were evaluated on Neuro2A cell line. Meanparticle size of all these nanocomplexes was lower than 220nm with surface chargeof 17.5 to 25.9 mV.Thelipopolyplexes, modified liposome and PEI 250KDa showed the highest transfection efficacy.Metabolic activity ofprepared vectors was 80-100% of control group. In conclusion, the prepared lipopolyplexes showed high ability toenhance gene transfer.

کلیدواژه ها:

Lipopolyplexes ، Non-viral vector ، Polyethyleneimine ، Transfection efficiency ، Gene delivery