A novel microextraction fiber coated by graphene oxide/silica nanocomposite on the surface of a cotton yarn for determination of nicotine from tobacco samples by HS-SPME method

A novel solid-phase microextraction (SPME) fiber was prepared by graphene oxide/silica (GO/ SiO2) nanocomposite as a novel stationary phase on the surface of a cotton yarn as a support using sol-gel technology. The nanocomposite structure was characterized by scanning electron microscopy (SEM) and Fourier transfer infrared spectrometry (FT-IR). The GO/ SiO2 nanocomposite coating, compared with commonly commercial fibers, showed better thermal and mechanical stability, longer durability, larger capacity, and higher extraction efficiency for nicotine. The GO/SiO2 coated fiber was used for determination of nicotine from tobacco samples using headspace solid-phase microextraction (HS-SPME) method, followed by gas chromatography-flame ionization detection (GC-FID). Different affecting experimental variables on the extraction efficiency, including extraction time and temperature, humidity and desorption conditions were evaluated and optimized. Under the optimal experimental conditions‚ the limit of detection (LOD), linear dynamic range (LDR), and relative standard deviation (RSD) for six repetitive extractions of 10 μg g-1 of nicotine were found to be 0.01 μg g-1, 0.8-30 μg g-1 (R2 = 0.996), and 6.9%, respectively. Finally, the GO/ SiO2/Cot was successfully applied for determination of nicotine in tobacco samples and the results showed good agreement with those obtained by a certified standard method.

کلیدواژه ها:

Headspace solid-phase microextraction (HS-SPME) ، graphene oxide/silica (GO/ SiO2) ، Cotton ، Nicotine ، Tobacco