Salinity response pattern of glyoxalase I gene from halophyte plant Aeluropus littoralis

Aeluropus littoralis (Gouan) Parlatore is a rhizomatous perennial halophyte monocotyledonous that can withstand environmental stresses such as salinity. In this study, the relative expression of glyoxalase I (GLY I) gene, as one of the main factors causing stress tolerance, were investigated in A.littoralis under salinity and recovery conditions by Real-time PCR. A.littoralis seedlings towards this purpose were subjected gradually to salt stress using 600 mM NaCl. Plant samples, were prepared, separately from the shoot and root tissues after 6 h, 24 h and 1 week behind the salt treatment, as well as 6h, 24h and 1 week after stress removing. Real-Time PCR was performed, in order to the GLY I expression level assessment. The results suggested significant increases of the GLY I expression levels, that were more remarkable among salt stressed treatments compared to the recovered samples. Maximum GLY I activities were observed after 6 and 24h salt stress that were 6 and 3.5 fold higher, respectively, compared to the control. Also these increases were more greater in shoot tissues than root ones. Overexpression of GLY I under NaCl stress, in fact was considered as a salinity tolerance factor in halophyte A.littoralis.

کلیدواژه ها:

Aeluropus littoralis ، Glyoxalase I ، Salinity tolerance