Optimizations and characterizations of Biosynthesis of Silver nanoparticles using leaf aqueous extract of Cannabis Sativa L

The plant extracts have been widely used as herbal reducing agents for green, easy, non-toxic and environmentally friendly nanoparticles synthesis. In this study, the extract of Cannabis sativa L. was used for synthesis of silver nanoparticles. The influences of various parameters including pH, volume of extract, extract concentration, concentration of silver nitrate and reaction times on the synthesis of silver nanoparticles were investigated [1]. The synthesized silver nanoparticles were characterized by some techniques such as; Transmission Electron Microscopy (TEM), X-Ray Diffraction (XRD), Fourier Transform Infra-Red (FT-IR) and UV-Vis spectroscopy. Afterwards, the antibacterial activity of these nanoparticles against human pathogenic bacteria was studied. The results showed that, the Ag NPs were spherical shape with particle sizes in the range of 5-22 nm. They also show maximum absorption at 420 nm due to Surface Plasmon Resonance (SPR). The optimum conditions for the synthesis of Ag NPs were determined as: pH = 8, 3 ml of extract, 0.5% concentration of extract, 1 mM of silver nitrate salt concentration and the reaction time of 20 minutes. Moreover, it was revealed that alkaloids and secondary metabolites act as both stabilizing and reducing agents. The Ag NPs showed a significant antibacterial activity

کلیدواژه ها:

Biosynthesis ، Cannabis Sativa L. ، Silver nanoparticles ، Surface Plasmon Resonance