VITAMIN A SUPPLEMENTATION AND THE GENE EXPRESSION OF T HELPER 1 CELL-RELATED CYTOKINE (IFN-Γ) AND TRANSCRIPTION FACTOR (T-BET) IN PATIENTS WITH MULTIPLE SCLEROSIS
سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 298
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شناسه ملی سند علمی:
INC15_037
تاریخ نمایه سازی: 30 دی 1397
چکیده مقاله:
Background and Aim: Multiple sclerosis (MS) is a chronic inflammatory neurological disorder of the human central nervous system (CNS) which is characterized by by Th1-Th2 cells imbalances, demyelination and consequently irreversible axonal damage. Vitamin A can suppress differentiation of immature CD4+ T cells into Th1 cells and shift the axis towards Th2 production. Methods: Thirty six patients randomly clustered into the intervention group receiving 25,000 IU/day vitamin A and the placebo group for 6 months. The proliferation and gene expression of IFN-γ and T-bet was evaluated in fresh, MOG-stimulated, PHA-stimulated and non-stimulated cells of patients by BrdU and Real Time-PCR respectively. Results: The results demonstrated that the IFN-γ expression in fresh (p=0.002), MOG-Stimulated (p=0.001) and non-stimulated (p=0001) had a noticeable reduction in the group receiving vitamin A. On the other hand, the gene expression of T-bet only in fresh and non-treated cells had a remarkable decrease in vitamin A-intake group (p=0.0001). Regarding PHA-treated cells, in contrast to T-bet expression which had a significant change in intervention group (p=0.007), the gene expression of IFN-γ did not decrease (p=0.485).Conclusion: Present study revealed that vitamin A supplementation reduced the gene expression of T-bet and IFN-γ in fresh, MOG-stimulated and non-stimulated cells in MS whether in PHA-treated cells, adverse other cells, the these genes were up regulated in placebo and intervention groups.
کلیدواژه ها:
نویسندگان
Maryam Safabakhsh
School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences
Ali Akbar Saboor Yaraghi
School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences
Niyaz Mohammadzadeh Honarvar
School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences
Mina abdolahi
School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences