Diagnostic value of RASSF1A gene hypermethylation in differential diagnosis of benign and malignant thyroid tumors

Introduction: Fine Needle Aspiration (FNA) is the best method for diagnosis of thyroid tumors, but it use only for about 20% of cases, the test results is reported as suspicious or intermediate. The aim of this study was to evaluate the RASSF1A gene hypermethylation as a Sensitive and Specific Molecular Test in Differential Diagnostic between Malignant and Benign Thyroid Tumors.method :To investigate hypermethylation of DNA after bisulfite treatment and PCR, enzyme digestion products are placed under. The measured concentration band in polyacrylamide gel (using software ImageJ) calculation of the quantity of methylation abnormalities. Further, the statistical analysis was used to determine the sensitivity and specificity of this test.Results: Quantitative evaluation of the RASSF1A gene hypermethylation 8 out of 25 samples malignant tumors from benign distinguish.Conclusion: The RASSF1A gene promoter hyper methylation has 92% sensitivity 100% specificity.

کلیدواژه ها:

Cardiovascular Diseases ، nutritional genomics ، Dietary pattern ، 9p21 ، Obesity