Association between TERT- CLPTM1L Gene to risk of cancer by rs401681(C) and rs2736098(A) in Iranian population

Abstract Cancers are considerably caused by a complex interaction between genetic and environmental factors. Highly penetrant mutations explain only a small fraction of cancer cases, while the majority of genetic cancer risk occurs due to contribution of many common sequence variants of low penetrance. It has been shown that two markers rs401681(C) and rs2736098(A) are in linkage disequilibrium (LD) with two genes TERT and CLPTM1L in which variation in the genomic region of the genes are associated with risk of many types of cancer. The Rs401681 resides in an LD block that contains the CLPTM1L gene and the 5′ end of the gene. The Rs2736098 is, also, linked to TERT-CLPTM1L genes that are causal genes for cancers in malignant tissues. In this study, we are testing the joint effect of rs401681(C) and rs2736098(A) in a population consisted of 100 individuals with different cancer types and 100 healthy individuals as control treatment. Therefore, DNA is extracted for each individual by protocol of kit, then PCR is ran according to the TETRA-ARMS method. Results of PCR are visualized after gel transferring to the Gel Doc to analysis the length of elongated DNA. The initial result shows polymorphic pattern for the used markers between patient and control populations, suggesting a genetic association between the genes and risk of cancer. Furthermore, the two markers might be used for marker assisted selection in order to detect risk of cancers in human populations.

کلیدواژه ها:

Cancer ، SNP ، CLPTM1L ، TERT