Inhalation exposure of Graphene nanoparticle induced oxidative stress in Melanoma

1. With the ever-increasing use of Graphene nanoparticle in health-related and engineering applications, the hazardous risks of this material have become a major concern. It is well known that Graphene NPs accumulate with cytotoxic and genotoxic levels within vital organs. It has also been shown that treating cell cultures with Graphene NPs resulted in cell cycle arrest and increased apoptosis/necrosis.2. In this study, we investigated chronic exposed to Graphene NPs at the exposure chamber in mice. The animals were divided into two groups (control and exposed group to Graphene NPs at the concentration of 3 µg/m3 for 5 h/day,) in a whole-body inhalation chamber.3. Our results showed that exposure to Graphene NPs induced the hematological and biochemical changes. The target organs for Graphene NPs were the melanoma in the mice, respectively. Also, NPs increased reactive oxygen species (ROS) generation, lipid peroxidation (LPO), the collapse of mitochondrial membrane potential (MMP), decreased a level of reduced glutathione (GSH) and finally increased a level of glutathione disulfide (GSSG) in melanoma tissues. Our results suggest that exposure to Graphene NPs can induce oxidative stress in the tissue mentioned.4. These results suggest that exposure of researchers and workers with Graphene NPs probably increase a risk of respiratory, cardiovascular and cerebral disorders through oxidative stress. However, good ventilation, appropriate personal protective equipment and using of anti-oxidant compounds in daily diet of worker are suggested.

کلیدواژه ها:

Graphene NPs ، chronic exposure ، oxidative stress ، reactive oxygen spices