Down-regulated MicroRNA 148a expression as predictive biomarker in lung cancer

سال انتشار: 1396
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 550

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شناسه ملی سند علمی:

IPMCMED02_060

تاریخ نمایه سازی: 29 فروردین 1397

چکیده مقاله:

Lung cancer is one of the most common invasive and lethal cancers in the world. Among the various types of lung cancer, 80% are classified as non-small cell cancers (NSCLCs) and 20% are small cell lung cancer cells (SCLCs). Despite the recent advances in diagnostic methods, such as CT scan that led to early diagnosis still more than 70% of patients are detected in metastatic phase, so finding a method for early detection seems necessary. According to recent studies, miRNAs are considered as tumor suppressors and oncogenes. Therefore, changes in the expression of miRNAs play a crucial role in tumorigenicity and cancer progression. Recently, a subset of miRNAs known as epi-miRNA, can directly or indirectly affect epigenetic processes, which confirms the relationship between miRNAs and epigenetics. The family of miR-148/152 is one of the reported miRNAs in various malignancies. In this study, 40 patients with NSCLC and 40 control samples were considered. miRNA was extracted from 2 groups. Then valuation of miRNA expression was don by using qRT-PCR (normalization whit snord). Finally, statistical analysis was used to compare miRNA expresion between cases and controls.It has been shown that serum levels of 148a microRNA have been significantly reduced in NSCLC patients compared to control group. Onset and progression of lung cancer is the result of the genetic and epigenetic interaction. One of the most important epigenetic changes that has been studied extensively is DNA methylation. One of the epi-miRNAs that affect the expression of DNMT1 and methylation is miR-148a, so, due to the reduced expression of this miRNA in cancerous specimens, the role of this microRNA consider as a tumor suppressor. In summary miR-148a can be introduce as a new marker for early diagnosis.

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نویسندگان

Shima Rahmati

Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.

Mohammad ABDI

Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.

Alina Abdolahi

Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.