The cytotoxicity effect of Nigella Sativa extract and Thymoquinone on SW480 human colon cancer cell line

سال انتشار: 1396
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 454

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شناسه ملی سند علمی:

IPMCMED02_106

تاریخ نمایه سازی: 29 فروردین 1397

چکیده مقاله:

Background:Thymoquinone (TQ), a compound isolated from black seed oil (Nigella sativa), has been shown to exert biological activity on various types of human cancers The goal of this study was investigation of the toxicity effects of the whole N. sativa’s extract and pure TQ on SW480 human colon cancer cell line. Methods:TQ content of extract was determined by HPLC technique. MTT assay was performed to examine the toxic effects of whole extract and pure TQ. Results:We demonstrated that sub-cytotoxic doses of TQ (100µM) in 24 hours and 80µM in 48 hours and 40µM in 72 hours decreased SW480 cell viability by 50%. Nigella sativa extract inhibited cell proliferation with an IC50 of 2.5µl in 24 hours and 1.5µl in 48 hours and 1µl in 72 hours. The exact amount of Thymoquinine of Nigella sativa extract was 132.48 ppm) Part Per Million(Conclusion:In the present study, we found that TQ and Nigella sativa’s extract significantly reduced the viability of human colon cancer SW480 cells in a concentration- and time-dependent manner.

نویسندگان

Saeede Mokhtari

National Institute of Genetic Engineering and Biotechnology(NIGEB), Tehran, Iran

Forough Sanjarian

National Institute of Genetic Engineering and Biotechnology(NIGEB), Tehran, Iran

Hamidreza Soleimanpour Lichaei

National Institute of Genetic Engineering and Biotechnology(NIGEB), Tehran, Iran