Evaluation of the gene expression level of Beclin 1 and Atg10 in autophagy and in patients with acute lymphoblastic leukemia

Acute lymphoblastic leukemia is the most common cancer in children and juvenile, it is also seen in adults, but ratio is lower. Autophagy is a programmed catabolic process of the cell for destruction of damaged organs and this function is done with the assistance of lysozymes. Disruption of autophagy leads to abnormalities in cellular processes associated with cancer and acts like a double-edged sword. In some cancers, the expression of autophagy genes is reduced, while in some others, the expression of autophagy shows increment. In this study, we evaluated the expression of Beclin 1 and Atg10 genes in 50 patients with de novo ALL compared to 18 healthy subjects as control using relative-quantitative-RT-PCR. The majority of B-ALL patients showed a significant reduction in the Beclin1 and Atg 10 genes compared to control group (P <0.05) and also was not observed correlation between Beclin1 expression and Atg10 expression level in these patients (P =0.926) and (r =-0.013). Due to the reduced expression levels observed in the essential genes of autophagy in our ALL patients and also other studies done in this field, we supposed that deregulated autophagy can be involved in leukomogensis and also autophagy can be regarded as therapeutic target to get rid of cancer.

کلیدواژه ها:

Acute lymphoblastic leukemia ، Autophagy ، Gene expression ، Beclin1 ، Atg 10 ، RT-PCR