Kidney cancer cells monitoring using a novel CNTs-based biosensor

A facile metal- and enzyme-free nanobiosensor using a glassy carbon electrode (GCE) modified with multiwall carbon nanotubes and zein bio-nanoparticles (GC/ZBN/MWCNTs) has been constructed at low cost. The electrochemical studies were carried out by using cyclic voltammetry (CV), amperometry and linear sweep voltammetry. The results demonstrated improvements in both electrical conductivity and catalytic activity of CNTs as was expected [1] when zein protein was wrapped around them. The sensor provided attractive electrocatalytic performances for sensitive and selective detection of hydrogen peroxide (H2O2) within a linear concentration range of 0.049-29.36 μM H2O2 with a detection limit of 35 nM H2O2 in 0.10 M phosphate buffer (pH 7.2). Moreover, the cytocompatibility of the constructed electrode made it an appropriate nanobiosensor for monitoring the living cancer cells through nanomolar level detection of H2O2 [2] as it released from both human dermal fibroblasts (HDF) and renal cell carcinoma (RCC; kidney cancer cell).