Evaluation of insulin resistance in murine polycystic ovary model

Background: Polycystic ovary (PCO) syndrome is one of the conditions resulting in miscarriage and other complications of women s reproduction system. Type 2 diabetes, obesity, metabolic syndrome, hypertension and so on are considered as risk factors of PCO syndrome. Metformin is used for the treatment, because of insulin resistance in such patients. Since insulin resistance in previous murine models of PCO has not been evaluated before, we are trying to approve this research modeling through empirical administration of insulin glargine.Methods: A number of 22 female and 7 male mice were divided into 4 groups; PCO modeling group, 2mg/100g testosterone enantate solved in olive oil; female control group, 1U/18g insulin glargine; male control group, 1U/18g insulin glargine; and a female group for combined administration of testosterone enantate and insulin glargine for drug interaction evaluation. All the administrations were daily for 2 weeks. Death in 1st 48h of stating insulin administration was considered as insulin sensitivity. Fisher s exact test was used for statistical analyses.Result: In the combined administration group, all the mice died after 1st administration. In the PCO modeling group, all the animals were survived after 2 weeks of administration of testosterone enantate, and also survived after empirical administration of insulin. Three out of 7 animals in the female controls, died in the 1st 48h. All the male controls, were survived after insulin administration. Insulin resistance in the modeling group was higher in comparison to the female controls, but it was not statistically significant (P=0.19).Conclusion: From the power analytic statistical point of view, further evaluations can verify the insulin resistance in murine models of PCO.

کلیدواژه ها:

Insulin ، Mice ، Polycystic ovary syndrome