Effect of royal jelly on the relative expression of GDF-9 BMP-15 genes on in vitro maturation of sheep oocytes
محل انتشار: سومین کنگره بینالمللی تولیدمثل
سال انتشار: 1396
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 411
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شناسه ملی سند علمی:
ISERB03_078
تاریخ نمایه سازی: 11 خرداد 1397
چکیده مقاله:
Background: This study was conducted o investigate the different percentages of royal jelly on in vitro maturation of sheep oocyte.Methods: Sheep ovaries were collected from local slaughter house and transferred to the laboratory via term-flask containing warm saline (35˚C) with 100 IU/ml of penicillin. COCs were recovered from antralfollicles (2-6 mm) by slicing method and cultured in to the medium 199 containing 10% FBS, 0.01 U/ml o FSH, 0.2 mM pyruvate and 0.2 mM glutamine and in a different percentages of royal jelly (0 , 0.25 , 0.5 and 1%). COCs cultured in maturation medium reached to MII after 24 hours. Data were analyzed as a compeletly randomized design with 4 treatments and 4 replicate in each.Result: The results of this study demonstrated that the addition of royal jelly to medium increased the rate of oocyte maturation (p Conclusion: The results indicated that the increase in percentages of different royal jelly increased significantly in vitro maturation oocyte rate from the control (61%) than the one percent(89%) in (p
کلیدواژه ها:
نویسندگان
Elahe Zarini Gakiyeh
Department of biology science Islamic Azad University, Qaemshahr, Iran
Hamid Deldar
Department of Animal Science, Sari Agrricultural Science & Natural Resources University, Sari, Ian
Rohollah Abdolahpor
Department of Animal Science, Islamic Azad Qaemshahr Agricultural Science and Natural Resources University, Qaemshahr, Iran.