The potential of innovative and novel therapeutic approaches through manipulation of non-codingRNAs for differentiation of MSCs to oocytes

سال انتشار: 1396
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 379

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شناسه ملی سند علمی:

ISERB03_094

تاریخ نمایه سازی: 11 خرداد 1397

چکیده مقاله:

Background: Approximately, 15-25% of couples suffer from infertility problems. In adult human ovaries, with age, the quantity and quality of oocytes is declining, and also delayed process of fertilization occurs. Proliferation and differentiation of germ cell are done by transcriptional regulation of specific genes. So far a few number of non-codingRNAs have been known and their functions of them are not clearly identified. RNAseq is one of high-throughput technologies of next-generation sequencing. By using this technology, we can evaluate whole transcriptome as well as RNAs that involved in stem cells differentiation pathways into oocytes with emphasis on non-coding RNAs.Methods: After culture and differentiation of mesenchymal stem cells into oocytes, in specific days of differentiation, the levels of the transcripts including ncRNAs were evaluated by using NGS technology. Also Real-Time PCR performed in order to investigate differentiation markers as well as confirmed data from NGS. We used some databases such as NONECODE ،LncRNADisease, GermlncRNA ، DIANA-LncBase, lncRNAdb, NRED, mirBase, mirwalk, mircode, mirdb for further analysis.Result: The results of Real-Time PCR showed that oocyte-specific markers upregulate during differentiation and downregulation of stemness markers. This study showed the expression changes of some transcripts including non-coding RNAs. Conclusion: Regarding up-or downregulation of non-coding RNAs during differentiation, we suggest that manipulation of some ncRNAs maybe a novel and innovative strategy for stem cell-based therapies in infertility treatment.

نویسندگان

Samira Ghasemi

Student Research Committee, Golestan University of Medical Sciences, Gorgan, Iran

Mohammad Shafiee

Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran