Histological evaluation of testis in male mice following torsion-detorsion of testis by mesenchymal cells and crocin

Background: In this study, the effect of mesenchymal cells (MSCs) and crocin (Cr) on the histological structure of testes following torsion-detorsion were evaluated.Methods: MSCs were isolated from bone marrow of the mice. 24 male mice were randomly divided into 4 groups and torsion-detorsion (TD) was performed at 720 degrees’ counter clock wise for half an hour in all groups, and injection of MSCs was performed in rete-testis. 1- Control group (C): In this group PBS was injected in left testes. 2- MSCs group: MSCs (5×105 in 10μL) was injected into the left testis. 3- Cr group: All mice received Cr 200mg/kg (IP). 4- MSCs+Cr group: All mice received MSCs along with Crocin. After 35 days, the testis of the mice was removed and fixed in a 10% buffered formalin and after tissue processing, paraffin sections were stained with H&E for histological investigations.Result: The results showed that the mean number of active Sertoli cells and Leydig cells in group MSCs + Cr was significantly increased in comparison to other groups (PConclusion: This histomorphometric study showed that the MSCs along with Cr were more effective in amelioration of testicular torsion damage.

کلیدواژه ها:

Crocin ، Mice ، Torsion ، Mesenchym