The effect of troxerutin administration during pubertal period on total volume of germinal epithelium of seminiferous tubules in type I diabetic rats

Background: Basal generation of ROS is essential for male reproductive function and identify as an independent biomarker of male infertility in diabetic patients. Troxerutin or vitamin P4 is a flavonoid that can be found in tea, coffee and cereal and has many pharmacological properties such as anti-inflammatory and antioxidant activities but there is no data about its supportive effects on fertility of adult male diabetic peoples.Methods: Fifty pubertal (8 weeks old) male wistar rats were divided into five groups including: Control, Diabetic (DM), TX, DM+Insulin and DM+TX. Type I diabetes were induced by a single dose of streptozotocin (IP, 55 mg/kg). Treatment groups received troxerutin (OG, 150 mg/kg/day) and insulin (SC, 4-6 IU/day) for 4 consecutive weeks. Finally, animals euthanized and then left testes were fixed in buffered formalin 10%. The samples were processed by standard paraffin embedding and serially sectioned at 20 μm thickness. Twenty to twenty-five sections per animal were selected through systematic random sampling and stained by H&E. A total volume of testis was calculated by Scherle s method and volume density of germinal epithelium (VGE) was estimated by point- counting and Cavalieri method by stereo-investigator system using an unbiased study.Result: Stereolocal results indicated that diabetes decreased VGE significantly (pConclusion: Therefore, it can be concluded that administration of TX is a suitable protective strategy to inhibit side effect of diabetes on seminiferous tubules of pubertal diabetic males.

کلیدواژه ها:

Diabetes ، Germinal epithelium ، P4 ، Pubertal ، Testis ، Troxerutin