Histological and histochemical study of prostate gland of mice with phenylhydrazine-induced hemolytic anemia; ameliorating effect of royal jelly

Background: Phenylhydrazine (PHZ) is used to prepare indoles and to form phenylhydrazines of natural mixtures of simple sugars. However, it is used to induce experimental anemia in animal models. On the other hand, royal jelly is known as antioxidant compound. Therefore, here in present study we aimed to evaluate the protective effect of royal jelly on PHZ-induced damages on ventral prostate.Methods: Adult male mice were put into four groups included eight in each group randomly. The first group received 0.1 ml normal saline. The second group received 60 mg/kg, IP of PHZ in 48 hour intervals. The third group received 100 mg/kg of royal jelly orally along with phenylhydrazine orally. The fourth group received similar doses of royal jelly. After 35 days, ventral prostate removed for histological and histochemical study using Hematoxylin and Eosin (H&E), Periodic Acid Schiff (PAS), Masson’s trichrome methods.Result: Negative effects of PHZ were seen in histological structure of ventral prostate. Exposure of prostate tissue to PHZ caused disorders in secretion, reduction of epithelium height and reduction of folding in wall of alveolus. On the contrary, groups receiving royal jelly showed improvement in prostate tissue structure and increased active alveolar and tubular units.Conclusion: In summary, results of the current study shows that royal jelly is an antioxidant agent and can be considered as an appropriate compound in order to inhibit anemia-induced damages on histological structure of ventral prostate.

کلیدواژه ها:

Histochemical ، Royall Jelly ، Ventral Prostate ، Phenylhydrazine