Investigation the effect of Achillea millefolium inflorescences extract on DNA damage of sperms in cyclophosphamide treated mice

سال انتشار: 1396
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 381

نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

ISERB03_219

تاریخ نمایه سازی: 11 خرداد 1397

چکیده مقاله:

Background: Apart from being an immunosuppressant agent, cyclophosphamide (CYP) has been known to induce oxidative stress, impact on gonadal cells’ DNA and reduce the fertilizing potential. Therefore, the present study was aimed to evaluate the effects of hydro-alcoholic extract of Achillea millefolium inflorescences (AMI), as a potential antioxidant on DNA damage in CYP treated mice.Methods: Thirty male adult NMRI mice were randomly arranged into 5 groups. Group 1 received normal saline (0.1 ml/kg), group 2 received CYP alone (5mg/kg), group 3 received CYP (5mg/kg) + hydro-alcoholic extract of AMI (75mg/kg). Group 4 received CYP (5mg/kg) + hydro-alcoholic extract of AMI (150mg/kg) and Group 5 received CYP (5mg/kg) + hydro-alcoholic extract of AMI (300mg/kg). Treatments were continued for 35 days. At the end, after mice euthanization by cervical dislocation, Caudaepididymis were used to collect sperm cells and rate of DNA damage were examined by Acridine Orange Staining. Statistical analyses were performed using ANOVA and Tukey test.Result: In group 2, the DNA damage significantly increased compared to control group (pConclusion: These findings indicated that AMI (low dose) has protective effect against CYP-induced toxicity in CYP treated mice probably by decreasing oxidative stresses. But, high dose of AMI caused increase toxicity of CYP.

نویسندگان

Neda Asle Iranifam

Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Shapour Hasanzadeh

Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Saeede Amani

Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Gholam Reza Najafi

Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.