Platelet reach fibrin scaffold causes reduction of inflammation and improves the endocrine function of autografted mice vitrified ovaries

Background: Anticancer treatments damage the reproductive and endocrine functions of the ovaries. Ovarian transplantation is a promising approach to preserve fertility in cancer patients. Platelet reach fibrin (PRF) facilitates angiogenesis and decreases inflammation reducing post transplantation damage in the ovaries. The aim of this study was to investigate the effect of ovarian tissue encapsulation in PRF on the inflammation and endocrine function of mice vitrified autografted ovaries in the gluteus superficial muscle.Methods: NMRI mice(4-5 weeks age) were divided into three groups(n=6): control (freshly isolated ovaries), vitrified autografted +PRF (ovaries were encapsulated in PRF just before transplantation), and vitrified autografted. 7 days after ovarian transplantation, the starting day of the estrous cycle was determined and 28 days following transplantation, the serum concentrations of progesterone, estradiol, TNFα, IL6 and IL-10 were measured. Data were analyzed using one-way ANOVA and Tukey s test and the means were considered significantly different at pResult: The levels of estradiol and progesterone and IL10 decreased significantly in the vitrified autografted group compared to the control. While the above parameters increased significantly in the vitrified autografted + PRF group to the control level. The estrous cycle recovery rate, IL6 and TNFα levels also increased in vitrified autografted group compared to the control and decreased in the vitrified autografted + PRF group significantly to the control level.Conclusion: Ovarian tissue encapsulation in PRF could effectively limit inflammation, improve restoring the endocrine function of autografted ovaries.

کلیدواژه ها:

Mice ، Ovary ، Platelet reach fibrin ، Vitrification ، Autotransplantation