Molecular genetic analysis of TGFβ in Iranian women with Endometriosis

Background: Endometriosis is a gynecological disorder which characterized by presence of endometrial tissue outside the uterus. Prevalence is estimated to be 6–10% in the general female population and 35–50% of the patients who labor from pain and/or infertility. Genetic, endocrine, immunological and environmental factors have critical role in its pathogenesis. Transforming growth factor beta (TGFβ) is one of the cytokines that play a role in fibrosis and angiogenesis and affects the immune responses. The aim of the present study was to assess the role of the TGFβ -509C/T polymorphism in susceptibility to endometriosis.Methods: In this study two groups of samples was evaluated: the endometriosis women (n=100) and non-endometriosis women (n=200) as control group. Genotyping of the TGFβ gene polymorphism at -509C/T was performed by Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).Result: The frequency of the TT, CT and CC genotype were 21%, 44%, 35% in patients and 16%, 50%, 32% in control group. No significant genotype distribution was observed between two groups.Conclusion: No statistically significant association was observed between TGFβ gene polymorphism -509C/T and the risk of endometriosis in Iranian women. So evaluation of other TGFβ variants is recommended.

کلیدواژه ها:

Endomertiosis ، PCR-RFLP ، Polymorphism ، TGFβ