The protective effect of allium cepa spermatogonia in alcohol – treated rats: A stereological study

Background: Ethanol exposure is known to suppress male reproductive activity in laboratory animals and humans. Onion (allium cepa) possesses many biological and pharmacological activities and is used widely in Iranian traditional folk medicine. The aim of this study was to evaluate the protective effect of onion juice supplementation on spermatogonia cells in alcohol treated rats.Methods: In this experimental study, 20 adult male rat were randomly divided into 4 groups (n=5) and treated for 30 days: control (1ml/kg; distillid water), alcohol (1ml/kg; ethanol 25% v/v), alcohol (1ml/kg)+ onion (3ml/kg; fresh juice) and onion(3ml/kg). All groups were given treatment orally. At the end of period, the rats were anesthetized with ketamin & xylaine and testes were removed and fixed in 20% neutral buffered formalin.The samples stained by H&E. The number of spermatogonia was estimated by unbiased stereological technique using optical fractionator and the data were analyzed using one way ANOVA.Result: The result showed that alcohol caused reduction significantly (pConclusion: It can be concluded that onion can be protective or ameliorate adverse effect of alcohol in testis and infertility of male alcoholic people.

کلیدواژه ها:

Allium cepa ، Rat ، Spermatogonia ، Alcohol