The role of Treg and Th17 cells in unexplained recurrent spontaneous miscarriage

سال انتشار: 1396
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 354

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شناسه ملی سند علمی:

ISERB03_319

تاریخ نمایه سازی: 11 خرداد 1397

چکیده مقاله:

Background: The main cause of unexplained recurrent spontaneous miscarriage (URSM) might be caused by inappropriate immunological responses of mother. We aimed to investigate the percentage of T helper 17 (Th17), T regulatory (Treg) cells and their cytokines in peripheral blood lymphocytes (PBL) of 20 women with URSM compared to 20 normal non-pregnant women (NNP) during luteal phase in window of implantation.Methods: Expression of Treg and Th17 related cytokines was measured using quantitative real-time PCR (qRT-PCR) method. The frequency of Th17 and Treg cells was evaluated by flow cytometry analysis. Study was approved by the Research Ethics Committee of Iran University of Medical Sciences.Result: The percentage of Treg cells in URSMs was significantly lower than NNPs (p=0.001). The percentage of Th17 cells in URSMs was higher than NNPs (p=0.01). IL-23, IL-17 and IL-6 expression levels in URSMs were significantly higher than NNPs (p=0.0001, p=0.038, p=0.05, respectively), but the higher expression levels of IL-21 and IL-10 were not significant. The expression levels of TGF-β, FoxP3, CTLA-4 and GITR in URSMs were lower than NNPs (p=0.001, p=0.001, p=0.01, p=0.005, respectively). Significant positive correlations were found between the percentage of Th17 cells with IL-23 (p=0.002), IL-6 (p=0.05) and IL-17 (p=0.003) and between expression of IL-23 and IL-6 (p=0.003) and IL-17 (p=0.002). IL-6 gene expression showed a significant positive correlation with IL-17 (p=0.005). Moreover, CTLA-4 and GITR expression were significantly correlated (p=0.0001).Conclusion: Imbalance of Th17/Treg cells and the consequent changes in cytokine expression levels might be implicated in the pathogenesis of URSM.

کلیدواژه ها:

CTLA-4 ، IL-10 ، IL-17 ، IL-21 ، IL-23 ، IL-6 ، TGF-β ، Unexplained recurrent spontaneous abortion (URSM) ، GITR

نویسندگان

Bita Saifi

Department of Anatomical Sciences, Mashhad Medical Science Branch, Islamic Azad

SeyedAbdolrahim Rezaee

Immunology Research Center, Inflammation and Inflammatory Diseases Division, Mashhad University of Medical Sciences, Mashhad, Iran

Mehdi Mehdizadeh

Cellular and Molecular Research Center, Department of Anatomical Sciences, Iran University of Medical Sciences, Tehran, Iran

Rosita Vakili

Immunology Research Center, Inflammation and Inflammatory Diseases Division, Mashhad University of Medical Sciences, Mashhad, Iran