DROSHA Gene Polymorphism and Recurrent Miscarriage

Background: Unexplained recurrent pregnancy loss refers to three or more abortions before 20 weeks gestation that approximately occurs in about one percent of the total number of couples. Drosha and Dicer are important molecules that have been identified in the trophoblast cells that confirm the activeness of biologic processes in micro RNA. Single nucleotide polymorphism in the Drosha (rs10719;C> T) encoded gene might be to produce the dysfunctional enzyme which was involved in the implantation process and leads to the pregnancy loss.Methods: We were designed a case-control investigation in the 100 subjects with unexplained recurrent pregnancy loss and 100 women with at least one child and without historical abortion as a control group. After DNA extraction, we used PCR-RFLP technique, in order to clarify the association between Drosha polymorphism and recurrent pregnancy loss.Result: Our findings revealed that the frequency of genotypes in the Wild-type homozygote (CC), heterozygote (CT) and mutant homozygote (TT) of Drosha in patients and healthy women,8%, 49%, 43% and 3%, 70%, 27%, respectively.Statistical analysis was shown significant difference genotype frequencies between cases and control groups (p=0.033).Conclusion: The present study revealed that polymorphism of Drosha gene can be a predisposing factor for recurrent pregnancy loss and also can be considered as a molecular biomarker in forewarning of recurrent pregnancy loss.

کلیدواژه ها:

Drosha ، micro RNA ، Recurrent Miscarriage ، Recurrent pregnancy loss ، Polymorphism